VALIDATION OF PCR-RFLP TESTING METHOD TO DETECT PORCINE CONTAMINATION IN CHICKEN NUGGET

Molecular barcodes detect redundancy and contamination in hairpin-bisulfite PCR.

PCR amplification of limited amounts of DNA template carries an increased risk of product redundancy and contamination. We use molecular barcoding to label each genomic DNA template with an individual sequence tag prior to PCR amplification. In addition, we include molecular 'batch-stamps' that effectively label each genomic template with a sample ID and analysis date. This highly sensitive met...

A PCR-RFLP assay to detect and type cytolethal distending toxin (cdt) genes in Campylobacter hyointestinalis

Campylobacter hyointestinalis is considered as an emerging zoonotic pathogen. We have recently identified two types of cytolethal distending toxin (cdt) gene in C. hyointestinalis and designated them as Chcdt-I and Chcdt-II. In this study, we developed a PCR-restriction fragment length polymorphism (RFLP) assay that can differentiate Chcdt-I from Chcdt-II. When the PCR-RFLP assay was applied to...

development of a pcr assay to detect mycoplasma contamination in cord blood hematopoietic stem cells.

background and objectives: contamination of cell lines and biological products is one of the major problems of cell culture techniques. rapid detection of mycoplasma contamination in cell culture is an important part of quality control standards in related laboratories. the aim of this study was to evaluate the efficacy of pcr in detection of myroplasma as contaminants in cell cultures and othe...

Failure of a PCR screening method to detect MRSA.

Development of a PCR assay to detect mycoplasma contamination in cord blood hematopoietic stem cells

BACKGROUND AND OBJECTIVES Contamination of cell lines and biological products is one of the major problems of cell culture techniques. Rapid detection of mycoplasma contamination in cell culture is an important part of quality control standards in related laboratories. The aim of this study was to evaluate the efficacy of PCR in detection of myroplasma as contaminants in cell cultures and other...